摘要
目的建立HPLC法测定史司唑仑的有关物质。方法采用十八烷基键合硅胶为填充荆，甲醇-水（65：35）为流动相，检测波长223nm。结果史司唑仑在1．2～2．8μg·ml^-1浓度范围内呈良好的线性关系（r=0．9998），RSD为0．56％。检测限为0．02ng。结论本方法结果准确，可用于艾司唑仑有关物质的控制。
OBJECTIVE An HPLC method for determination related substaaces of Estazolam was established . METHODS A C18 column was used with the mobile phase of methanol-water（65 ： 35）, the detection wavelength was 223nm. RESULTS The linear range of Estazolam was 1.2-2.8μg · ml^-1 （ r =0. 9998）, RSD =0. 56%. The detection limit was 0.02ng. CONCLUSION The method was accurate,it can be used to determine related substances of Estazolam.
出处
《齐鲁药事》
2006年第11期660-662,共3页
qilu pharmaceutical affairs
