红外线及磁场对兔膝关节骨性关节炎氧化过程的影响

The influence of infrared radiation and magnetic fields on oxidation process of rabbit's knee osteoarthritis

目的:探讨红外线及磁场对兔膝关节骨性关节炎(OA)氧化过程的影响。方法:首先采用管型石膏伸直位固定右后肢的方法将24只健康雄性新西兰成年白兔复制出膝关节骨性关节炎模型。6周后将24只兔子全部解除石膏固定,并随机分为4组,即对照组、红外线治疗组(红外组)、磁疗组及红外线和磁场联合治疗组(联合组)。解除石膏后,即开始处理。分别于治疗开始后1、2、3周处死白兔,每组每周处死2只,处死前先行测量每只兔子的膝关节活动度(ROM),然后留取血清及关节灌洗液标本以备检测超氧化物歧化酶(SOD)及丙二醛(MDA)。结果:与其他各组比较,联合组兔OA模型的ROM有显著提高(P<0.05),其关节灌洗液中MDA的含量降低而SOD的含量增加(P<0.05),但血清SOD和MDA的含量却无明显变化(P>0.05);两个单纯性治疗组差异无显著性意义(P>0.05)。结论:联合应用红外线和磁场对于提高兔膝关节OA的ROM和关节局部的抗氧化能力有较好疗效,其治疗效果要明显好于单一物理因子的治疗。

Objective：To discuss the effect of infrared radiation and magnetic fields on oxidation process of experimental knee osteoarthritis（OA） of rabbit model.Method：Twenty-four New Zealand grown rabbits were duplicated for OA model of the knee of rigbt hindlimb by extended fixation technique,and after 6 months,all the rabbits were relieved from fixation.The rabbits were randomly divided into four groups： control group （no treatment） , infrared group （treated with infrared radiation）,magnetotherapy group （treated with magnetic fields） and the combined therapy group （treated with infrared radiation and magnetic fields）. At the ends of the first,second and third week after treatments,two rabbits in each group were taken to measure the range of motion （ROM） of fixed knee joints before executed. Then,the serum and douche fluid of all the fixed joints were obtained for the testing of superoxide dismutase （SOD） and malondialdehyde （MDA）.Result：Compared with other groups,ROM of fixed knee joints improved significantly （P〈0.05） and the content of SOD in articular douche fluid increased while MDA declined in combined therapy group （P〈0.05）. There was no significant difference between the two single therapy groups （P〉0.05）.Conclusion： Treatment of infrared radiation combined with magnetic fields might have better curative effects at improving ROM and local anti-oxidation ability of OA joints.