两个Y染色体短串联重复序列基因座的遗传多态性

Polymorphic analysis of two Y chromosome short tandem repeat loci in Chinese Chengdu Han population

目的调查DYS508、DYS516两个Y染色体短串联重复序列基因座的遗传多态性在成都汉族群体中的分布。方法用PCR扩增、非变性聚丙烯酰胺凝胶电泳分型;构建等位基因分型标准物;对所有等位基因测序,按照国际法医遗传学会原则命名等位基因。结果获得DYS508、DYS516基因座等位基因个数分别为6、6;基因变异度分别为0.7242、0.7931;单倍型变异度、个人识别能力和非父排除率均为0·9397。结论DYS508、DYS516这两个Y染色体短串联重复序列均具有较好的遗传多态性,可用于法医学的混合斑分析和父系亲属间的亲权关系鉴定。

Objective To reveal the distribution of genetic polymorphism of two novel Y chromosome short tandem repeat （Y-STR） loci （DYS508, DYS516） in Chinese Chengdu Han population. Methods Two Y-STR loci were amplified with PCR. The products of PCR were analyzed with polyacrylamide gel electrophoresis. Each allele of these 2 Y-STR loci was sequenced and the allele ladders were constructed. Alleles of these Y-STR loci were nominated according to recommendations of the International Society of Forensic Genetics （ISFG）. Results Six and 6 alleles were observed in loci DYS508 and DYS516 respectively. The gene diversity of DYS508 and DYS516 was 0.7242 and 0.7931 respectively. The diversity of haplotype for these Y-STR loci was 0.9397. The value of discrimination power and the exclusion chance of paternity for these Y-STR loci were same as the value of haplotype diversity. Conclusion Both Y-STR loci are high genetic polymorphic. They are benificial to the test of male-female mixtures and paternity identification in forensic science.