中西药结合促进心梗后大鼠胆碱能神经的恢复

Restoration of cardiac cholinergic innervation in the rat after myocardiac infarction by adopting traditional chinese and western medicine

目的:观察葛根素与神经生长因子联合应用对大鼠心肌梗死后去胆碱能神经支配的影响,以证实二者联合应用对心梗后大鼠胆碱能神经功能恢复的作用。方法:实验用SD大鼠32只,分为正常组、心肌梗死组、神经生长因子组及联合用药组。术后2d取材,以Karnovsky-Roots法,显示胆碱能神经纤维,应用多功能真彩色病理图像分析系统分析胆碱能神经纤维密度。结果:心肌梗死组存活心肌中胆碱能神经纤维密度最低,联合用药组胆碱能神经纤维密度最高。结论:葛根素与神经生长因子联合应用能够明显减缓大鼠心肌梗死后的去胆碱能神经支配,促进神经功能的恢复。

Objective： To confirm the functional restoration of cholinergic innervation after acute myocardial infarction （AMI） in the rat by using both puerarin （Pue） and nerve growth factor （NGF）. Methods： 32 SD rats were used in this study. They were divided into normal, AMI, NGF and medicine-combined groups. The samples were taken two days after the operation. Karnovsky-Roots method was used to show the cholinergic fibers. The density of positive fibers was calculated by Medical Pathological Image Analysis Processing System. Results： There was the least density of cholinergic fibers in AMI group, and the most in medicine-combined group. Conclusions： The treatment combining Pue and NGF has the effect of decreasing the control of denervation greatly after AMI in the rat, and it may promote the functional restoration of cholinergic nerve.