亚硒酸钠溶液诱导胃上皮肿瘤SGC-7901细胞凋亡的实验研究

Apoptosis of gastric epithelial tumor SGC-7901 cells by sodium selenite

目的：探讨亚硒酸钠能否诱导胃上皮肿瘤SGC-7901细胞凋亡，以及凋亡诱导与细胞周期改变的关系。方法：亚硒酸钠溶液不同浓度、不同作用时间分别处理SGC-7901细胞，应用电子显微镜观察亚硒酸钠溶液作用后细胞形态变化，应用DNA末端原位标记染色法（TUNEL）及流式细胞仪技术分析细胞凋亡及细胞周期的改变。结果：亚硒酸钠作用于SGC-7901细胞后，可看到较为典型的细胞凋亡的形态学变化：细胞核固缩、染色质凝集、呈新月型紧贴于核膜周边，核碎裂，染色质片段化，凋亡小体形成等。流式细胞仪DNA直方图上出现典型的亚二倍体的“凋亡峰”。TUNEL染色法细胞凋亡指数在8．4％～33．4％。结论：亚硒酸钠能够诱导SGC-7901细胞凋亡，亚硒酸钠诱导SGC-7901细胞凋亡与细胞周期阻滞密切相关。

Objective： To investigate whether sodium selenite can efficiently induce SGC-7901 cells, and to study the relationship between the apoptosis and the cell cycle. Methods： Being treated with sodium selenite under different concentrations and different times, SGC-7901 cells were observed the morphologic changes by electron microscope, and the apoptosis and the cell cycle by TUNEL method and flow cytometry. Results： After 12 hours of exposure to the drug, changes like cell shrinkage, nuclear condensation and formation of apoptotic bodies were exhibited. A typical sub-diploid peak before G0/G1 phase was observed by flow cytometry. The apoptosis index was 8.4% to 33.4% by TUNLE. Conclusion： Sodium selenite can induce the apoptosis of SGC-7901 cells, which is associated with the blockage of cell cycle.