表达γ-亚麻酸的无筛选标记转基因大豆的鉴定

Characterization of Marker-free Transgenic Soybeans with Δ~6-fatty Acid Desaturase Gene Expressing γ-linolenic Acid

对所获得的9株无筛选标记基因的T1代莆豆8008转基因大豆的T2代进行了分析。PCR和Southern杂交检测表明标记基因已不存在于转基因株系中,同时目的基因以纯合或者杂合状态存在于部分株系中,除草剂抗性分析也表明这些转基因株系已不再具有抗除草剂的能力。Northern杂交检测表明Δ6-脂肪酸脱氢酶基因在转基因株系中可以转录。GC分析表明其中6个转基因植株富含GLA。

Nine lines of T2 generation of marker-flee transgenic soybeans of Pudou 8008 with △^6-fatty acid desaturase gene were characterized. PCR and Southern blot indicated that the selective marker gene （bar） had been removed from some transgenic soybean progenies, while △^6-fatty acid desaturase gene was still in some progenies of these transgenic soybean lines. Northern blot showed that the △^6-fatty acid desaturase gene could be transcribed in the marker-free transgenic soybean lines. Gas chromatographic analysis showed that six transgenic soybean lines could produce γ-linolenic acid.