黄斑橡胶榕离体再生体系的建立

In vitro Culture and Plant Regeneration of Ficus robusta

以黄斑橡胶榕(Ficus robusta“Yellow spot”)的顶芽为外植体进行离体培养与植株再生研究。结果表明,黄斑橡胶榕的诱导培养基以MS+BA 2.0 mg L-1+NAA 0.1 mg L-1为宜;继代增殖以MS+BA 1.0 mg L-1+NAA 0.05 mg L-1为宜,每个外植体可产生3个芽;生根培养基以MS+IBA 0.1 mg L-1+AC 100 mg L-1为宜,生根率96.67%以上。试管苗移栽成活率达到98%以上。

Callus was obtained from the terminal bud explants of Ficus robusta on Murashige and Skoog （MS） basel medium supplemented with 2.0 mg·L^-1 BA and 0.1 mg·L^-1 NAA. Following subculture on MS medium supplemented with 1.0 mg·L^-1 BA and 0.05 mg·L^-1 NAA, each explant produced 3 shoots. The frequency of rooting was over 96.67% on MS medium supplemented with 0.1 mg·L^-1 IBA and 100 mg·L^-1 AC. The survive rate after transplant reached above 98%.