蓝猪耳受精生物学研究进展

Advances in Studies on Fertilization of Torenia fournieri(Scrophulariaceae)

蓝猪耳(Torenia fournieri L.)胚囊半裸露,在光学显微镜下能清楚观察到卵细胞、助细胞及部分中央细胞的形态结构,有助于原位观察卵细胞在受精前后的变化状态,被认为是研究被子植物体内受精机理的一种模式植物。综述了蓝猪耳的受精机理:花粉管定向进入胚囊的方式与机理、钙在受精过程中的作用、受精前后胚囊细胞骨架的动态变化。简要介绍了离体受精技术在蓝猪耳受精生物学中的发展应用。根据前人对蓝猪耳的研究成果并结合我们的研究,指出蓝猪耳在受精生物学中的应用,特别是借助离体受精技术平台,将具有更大的研究前景。

Toreniafournieri （Scrophulariaceae） is a suitable experimental plant for studies of angiosperm fertilization since it has semi-naked embryo sac within which egg cell, two synergids and part of the central cell are clearly visible under the light microscope, which facilitates the in situ observation of egg cell before and after fertilization. Progress in researches on fertilization mechanism of T. fournieri is reviewed in the following aspects： how the pollen tubes get into the embryo sac, dynamics of calcium in fertilization process, changes in the cytoskeleton of embryo sacs before and after fertilization. The significance ofT. fournieri in the studies of in vitro fertilization is briefly described.