血管紧张素转换酶和血管紧张素Ⅱ-1型受体基因多态性与冠心病的相关性分析

Association between angiotensin-converting enzyme and angiotensin Ⅱ type 1 receptor gene polymorphism and the coronary heart disease

目的 研究血管紧张素转换酶(ACE)基因I/D多态性和血管紧张素Ⅱ-Ⅰ型受体(AT_1R)基因A1166/C多态性与冠心病(CHD)的关系。方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测130例CHD组和90例对照组ACE和AT_1R基因多态性。结果 ACE-DD基因型频率在CHD组显著高于对照组(38.5％,14.4％,P<0.001)。AT_1R-AC基因型在两组间差异无显著性(13.1％,10％,P>0.05),但合并AC基因型的DD型患者发生CHD和MI的OR值(5.836和3.985)明显高于合并AA型(3.102和2.979)。结论 ACE基因I/D多态性中DD基因型是冠心病发病的独立危险因素之一,AT_1R-C等位基因增加ACE-DD型发生CHD和MI的危险,二者具有协同作用。

Objective To explore the relation of angiotensin-converting enzyme （ACE） and angiotensinⅡ type 1 receptor （AT1R） gene polymorphisms to coronary heart disease （CHD） and to search for interaction between the two polymorphisms. Methods The insertion/deletion of ACE gene polymorphism and AT1R gene polymorphism （an A→C transversion at nucleotide position 1166） were detected by polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP） in 130 CHD patients and 90 controls. Results DD genotype of ACE was more frequent in CHD patients than that in control group （38.5%, 14.4%, P〈0.001） . The frequency of the AT1R A/C genotype did not differ between the patients and the controls （13.1%, 10%, P〉0.05） . The relative risk of MI and CHD associated with the ACE-DD in patients with AT1R -AC genotype （5.836, 3.985） is higher than that in patients with AT1R -AA genotype （3.102, 2.979） . Conclusion DD genotype should be an independent risk factor for CHD and MI in Chinese people, the AT1R -C allele may increase the relative risk of MI and CHD associated with the ACE-DD, there is a synergistic interaction between the ACE-I/D and AT1R -A/C gene polymorphism.