摘要
目的:研究β纤维蛋白原启动区-455G/A基因多态性和血浆纤维蛋白原浓度与急性冠状动脉综合征(ACS)之间的关系。方法:将冠状动脉造影检查的229名患者,分为ACS组89例、稳定性心绞痛(SAP)及陈旧性心肌梗塞(OMI)组97例、对照组63例。提取基因组DNA,经多聚酶链式反应(PCR)加HindIⅢ内切酶技术检测目的基因片段;采用自动化检测系统检测血浆纤维蛋白原(Fg)浓度。结果:ACS患者血浆Fg水平明显高于对照组,ACS患者A等位基因频率高,多表现为AA、AG基因型,A基因携带者(GA+AA)均较GG基因型者血浆纤维蛋白原水平明显增高(P<0.05)。结论:BβFg-455G/A基因多态性可能对ACS的发生及病理生理变化有影响,并可影响血浆纤维蛋白原水平,其中A等位基因可能与冠心病的不稳定性有关。说明ACS患者急性冠状动脉内血栓形成有遗传因素参与,而且A等位基因可能是ACS的一个危险因子。
Objectives To investigate the relationship between the β fibrinogen gene -455G/A polymorphism and plasma fibrinogen level and to determine the influence of the acute coronary syndromes. Methods Eighty-nine patients with ACS, ninety-seven patients with SAP and OMI, and 63 control subjects. The β fibrinogen gene 455G/A polymorphism was analyzed by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） with the restriction enzyme Hae m, while plasma fibrinogen levels were obtained from the prothrombin time assay. Results The plasma fibrinogen levels were significantly higher in patients with ACS than that in tire controls （P〈0.01）. A allele frequency was higher in patients with ACS than patients with SAP and OMI, and control subjects. Higher plasma fibrinogen levels were shown in the subjects with A-alleles （AA ＋GA genetype） than the subjects with G-alleles （GG genetype） （P〈0.05）. Conclusion The promoter region of β fibrinogen gene 455G/A polymorphism is associated with the produce and development of ACS, and at the same time impact on the plasma Fg concentration. Tire A allele frequency in patients with ACS was significantly higher and the A allele gene may be a risk faelor for ACS.
出处
《中国分子心脏病学杂志》
CAS
2003年第6期323-325,共3页
Molecular Cardiology of China
