糖脂抗体和银杏制剂对体外培养雪旺细胞影响的研究

The effects of antiphospholipid antibodies and EGb761 on the Schwann cells in vitro

目的研究抗糖脂抗体对离体培养的雪旺细胞(schwanncell,SC)的影响和银杏制剂对其的保护作用。方法研究对象为1998年2月至1999年12月上海复旦大学附属华山医院神经内科自身免疫性周围神经病患者25例以及4名正常体检者。用饱和硫酸铵法提取血清球蛋白,加入离体培养的SC中,观察细胞形态改变,并通过单因素方差分析和配对t检验的方法分析细胞密度、周长和面积变化以及银杏制剂在其中是否具有保护细胞生长的作用。结果加入患者免疫球蛋白的SC形态发生显著改变,加入补体后细胞密度更显著下降。加入银杏制剂后细胞形态和密度无明显改变。结论自身免疫性周围神经病患者的免疫球蛋白在补体的参与下对体外培养的SC有明显的破坏作用,银杏制剂在这种情况下无显著的保护体外SC继续生长的作用。

Objective To detect the effect of the antiphospholipid antibodies on the Schwann cells in vitro and to find if the Extract of Ginkgo biloba 761 （ EGb761 ） has any protective effects. Methods The immunoglobulins （Ig） were extracted from the serum of the patient with autoimmune polyneuropathies and those with controls. Ig only or Ig plus calf serum （ served as complement） were added into the culture solutions of the Schwann cells. The density of the cell in one high power field, the cell perimeter and area were detected and compared to those blank and those controls. Results The shape of the Schwann cells in both the Ig and Ig plus calf serum solution were greatly changed. The densities of the cells in them were both lower than the blank ones, with more severe in the cells with calf serum. There was no significant different between the solutions with or without EGb761. Conclusion lg from the patients with autoimmune polyneuropathies could attack the Schwann cells in vitro, with the existence of complements, the destruction was even more severe. No protection of EGb761 could be found in these situations.