摘要
分析了影响农杆菌介导杨树转化效率的因素,如植物基因型、农杆菌类型、菌液浓度、侵染时间、共培养时间、Vir基因的活化、选择标记基因等,同时指出转化受体也是影响转化效率的重要因素。
The factors influencing transformation efficiency were discussed, such a.s different genotypes, Agrobacterium strains, Agrobactefium conccntration , time of infection, time of co-culture, activation of Vir genes, marker genes et al. And it was pointed out that gene transformation receptor was also an important factor influencing transformation efficiency.
出处
《安徽农业科学》
CAS
北大核心
2006年第22期5772-5773,5797,共3页
Journal of Anhui Agricultural Sciences
基金
大连民族学院人才引进科研项目启动基金(20046205)
关键词
杨树
农杆菌
遗传转化
Populus
Agrobacterium
Genetic tranformation