不同取胚时期对葡萄杂交胚培养的影响

Effect of Different Embryo-collecting Time on Hybrid Grape Embryos Culture

对无核白等4个葡萄品种的杂交胚在不同时期取胚培养,对胚发育和萌发结果进行曲线回归,通过回归方程和拟合曲线找出不同杂交胚生长发育的特点和规律。结果表明,不同杂交胚适宜的取胚时期各不相同。回归方程中常数越大,其对应的杂交胚越容易培养,其发育率和萌发率较高。取胚时期内,多数杂交胚发育和萌发的变化趋势表现为峰形曲线。

Hybrid grape embryos crossed among Thompson＇ s Seedless and 3 other varieties were cultured after being colleted in different time. In order to find the development characters of hybrid embryos, regression formulas and fitting curves were regressed out from embryo development and embryo germination. The result showed that different hybrid embryos need different embryo- collecting time. in the regression formulas, the bigger the constants are, the more easily corresponding embryos are developed. Majority development curve and germination curve display peak lines.