摘要
以C18为分离柱,以100mmol/L磷酸钠缓冲溶液(pH2.1)/0.8mol/L辛基磺酸和乙腈=9:1(v/v)混合液为流动相,检测波长为270nm的液相色谱检测VB1含量,在0.005μg￣2.5μg范围内进样与主峰面积线性关系良好(r=0.9997),最小检测量0.0050μg,检出限0.0002μg。
The content of vitamin B1 （VB1） was detected by high performance liquid chromatography （HPLC） at 270 nm, in which C18 Chromatography column was used. The mobile phase was a mixture containing acetonitrile and the solution of 100 mmol/L Na3PO4 （pH 2.1） and 0.8 mol/L octylsulfoacid, with the mix ratio of 1：9 （v/v）. The content of VBI and the area of main peak presented good linear relationship （r=0.9997） when the content of VBI ranged between 0.005 μg and 2.5 μg. The minimum injection dose was 0.0050 μg, and the detection limit was 0.0002 μg.
出处
《中国酿造》
CAS
北大核心
2006年第12期57-58,共2页
China Brewing
关键词
VB1
高效液相色谱法
含量
VB1
high performance liquid chromatography （HPLC）
content
