类风湿性关节炎患者滑膜液和外周血中细胞因子的检测和对比分析

A comparative analysis and detection of IFN-γ, IL-10, IL-4 and IL-12 in the synovium fluid and peripheral blood from patients with rheumatoid arthritis

目的探讨细胞因子在类风湿性关节炎（RA）发病中的作用。方法用酶联免疫吸附试验（ELISA）分析10例RA患者滑膜液和血清中干扰素γ（IFN-γ）、白细胞介素（IL）-10、IL-4与IL-12的含量。结果RA患者滑膜液中IFN-γ含量很高[（366.7±43．2）pg／ml]，而外周血中仅（20．1±3.2）pg／ml。患者滑膜液和外周血中IL-4含量均低于检测下限（〈15pg／ml）。患者滑膜液中IL-12和IL-10含量分别为（419．9±89．2）pg／ml和（187．7±34．5）pg／ml，均高于外周血中的含量[（65．32±34．2）pg／ml和（85．0±12．7）pg／m1]。结论RA患者关节滑膜液中的细胞因子分泌格局为Th1型，且明显高于外周血中含量，提示这些炎性细胞因子增高可能与关节滑膜中的自身反应性T细胞的活化导致的免疫损伤密切相关。

Objective To explore the effects of cytokines on the pathogenesis of rheumatoid arthritis（RA）. Methods The levels of IFN-γ, IL-10, IL-4 and IL-12 in the synovium fluid and peripheral blood of 10 cases of patients with RA were detected by enzyme-linked immunosorbent assay （ELISA）. Results The concentration of IFN-γ was （366.7±43.2） pg/ml in the synovium fluid which was much higher than that of serum [（20.1±3.2） pg/ml]. The concentration of IL-4 was very low （〈15 pg/ml） in both of synovium and serum. The concentration of other two cytokines, IL-12 and IL-10 were （419.9±89.2） pg/ml and （187.7±34.5） pg/ml respectively. The concentration of IL-12 was （65.32±34.2） pg/ml and IL-10 was （85.0±12.7） pg/ml in the serum. Conclusions By comparing with the concentration in synovium, the concentration of IFN-γ and IL-12 in the serum was significantly lower. These results demonstrated that the cytokine profile showed Thl type, an elevation of contents of IL-12 and IL-10 in the synovial fluid of patients with RA, and this might be associated with the pathogensis of RA.