HPLC法测定香菊感冒颗粒(无糖型)中刺槐素的含量

Determination of Acacetin in Xiangjuganmao Keli(no sweet) by HPLC

目的：建立高效液相色谱法测定香菊感冒颗粒（无糖型）中刺槐素的含量。方法：刺槐苷使用甲醇超声提取并用盐酸水解在、ODS（5μm，4．6×250mm）柱上，以甲醇-水-冰醋酸（350：150：2）为流动相进行分离，流速1．0ml／min，340nm波长检测。结果：方法准确、精密度高、重现性好，浓度在2．00-10．00μg／ml范围内线性关系良好，r=0．9998，平均加样加收率为99．9％，RSD=0．41％（n=6）。结论：方法简便、专属、重现性好，可作为香菊感冒颗粒质量控制的一种方法。

Objective： To establish a method for the determination of acacetin in Xiangjuganmao Keli （no sweet）. Methods： Acacetin in powdered herb was extracted by ultrasonator with methanol and was hydrolyzed with hydrochloric acid. Separation was accomplished on an ODS reversed phase column（5 μm, 4. 6 × 250 mm） with a mobile phase of methanol-water-acetic acid（ 350： 150： 2）. The detective wavelength was at 340 nm. Results： The method was accurate, the results were stable and reproducible. The linear range of calibration cure was within the concetration of 2. 00 - 10. 00 μg/ml （ r = 0. 9998 ）. The average extraction recovery was 99.9% （n =6）, RSD =0. 41% （n =6）. Conclusion： The method is simple, convenient, sensitive, and reproducible for quality control of Xiangjuganmao Keli（ no sweet）.