白英提取液对Hela细胞凋亡及Fas/FasL基因表达的影响

The Influence of Solanum lyratum Thunb Extract on Apoptosis and the Expression of Fas/FasL Genes in Hela Cells

目的:探讨白英提取液对Hela细胞凋亡及Fas/FasL基因表达的影响。方法:采用MTT法检测细胞增殖抑制率,流式细胞仪检测凋亡,二步法免疫组化检测Fas蛋白表达,半定量RT-PCR检测Fas和FasL mRNA表达。结果:白英提取液对Hela细胞增殖有较强的抑制作用,且这种作用呈一定的剂量-时间依赖性;细胞凋亡率明显升高;Fas基因mRNA和蛋白表达显著上升,FasL mRNA表达明显下降。结论:白英提取液通过上调Fas基因和下调FasL基因表达,诱导细胞凋亡,从而抑制Hela细胞增殖。

Objective ： To explore the effects of Solanum lyratum Thunb （SL） extract on the apoptosis and the expression of fas and fasL genes in Hela cells. Methods ： The proliferation inhibitory rate was evaluated by MTI＂ assay. Induction of cell apoptosis rate was determined by flow cytometry. The expression of Fas protein was detected by two-step immunhistochemical staining. The expression of fas and fasL mRNA was detected by semi-quantitive RT-PCR. Results： SL extract displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against Hela cell. The rate of apoptosis was increased obviously. The expression of fas mRNA and protein was increased significantly, and fasL mRNA was decreased markedly. Conclusion： SL can induce apoptosis by up-regulating expression of fas and fasL genes, and inhibit the development of Hela cells.