中药治疗肝纤维化大鼠对ALR基因表达的影响

Effect on ALR mRNA Expression of the Hepatic Fibrosis Rats Treated by Chinese herbs

目的:从分子水平探测中药治疗肝纤维化的作用及机制。方法:利用CCL4复合因素建立SD大鼠肝纤维化模型。实验动物分成正常对照、模型、中药治疗三组,分别检测各组生化、肝纤维化指标和肝组织中ALR mRNA表达变业。结果:1.ALT、AST、A/G、HA、PCⅢ、LN、PLD各指标:模型组均高于对照组(P<0.01),中药治疗组低于模型组(P<0.01),并且,HA、PCⅢ、LN、PLD的降低与病理组织反应肝纤维化的好转程度一致;2.肝组织中ALR mRNA的相对表达量:中药治疗组、模型组明显高于对照组(P<0.01),中药治疗组高于模型组(P<0.01)。结论:联联合检测生化、肝纤维化指标可提高肝纤维化早期诊断;肝纤维化时肝组织ALR mRNA的高表达可逆转肝纤维化。

Objective：To sutdy the molecular mechanism of treatment by Chinese herbs on the hepatic fibrosis of rats. Methods ： Hepatic fibrosis model of rats were established with CCL4 Then the rats were divided into control, model and Chinese herbs treated groups. The elevel of ALR mRNA expression, biochemical and hepatic fibrosis paparmeters were observed from different groups. Results： The concentrations of ALT,AST,A/ G,HA,LN,PLD and PCⅢ in serum are 1. model group higher than control （P 〈 0.01）,2. Chinese herbs treated group lower than model group（ P 〈 0.01 ）.