大鼠结肠癌腹腔转移模型的建立和肿瘤切除术后结肠吻合口羟脯氨酸含量的测定

Development of abdominal transfered colon carcinoma rats model and the concentrations of hydroxyproline in colon stoma tissue after tumorectomy

目的:建立一种新型的大鼠结肠癌腹腔转移模型,通过测定吻合口组织羟脯氨酸含量研究荷瘤大鼠行肿瘤切除术后吻合口的愈合情况。方法:人结肠腺癌细胞SW-480注射种植到裸鼠皮下,形成瘤体后将瘤块开腹移植到SD大鼠结肠黏膜下造成6例大鼠结肠癌腹腔转移模型,行肿瘤切除术。选取6只等体重SD大鼠为对照组,进行假手术。1周后测量各组吻合口抗张力,用高效液相法(HPLC)测量吻合口组织羟脯氨酸含量。结果:SD大鼠成瘤率为60%,并且成瘤大鼠均可见腹腔转移。荷瘤组吻合口张力和羟脯氨酸含量均显著低于假手术组。结论:该方法制作对模型稳定可靠,而肿瘤可显著延迟吻合口愈合。

Objective：To develop a new kind of abdominal translered colon carcinoma model in rats, and to evaluate the healing of stnma by measure the concentrations of hydroxyproline in tissue of colon stoma, Methods：Colon Carcinoma cell SW480 was inoculated subcutaneously into athymic mice tu develop an implantation tumor,Subsequently the tumor lumps were implanted to the SD rats＇ colon mucosa to setup the model. The tumor tissues were cut and the colons were anastomosed. 6 rats which were not implanted tumors but colons been cut and anastomosed were included into control group, Then measure the tensile strength and concentrations of hydroxyproline of colon stoma tissue with HPLC. Results：60% rats were success to establish abdominal transfered Colon Carcinoma models. The tensile strength and the coneentrations of hydroxyproline in colon stoma tissue were both lower in experiment group than in control group（P〈0.0S.） Conclusion：It＇s a reliable methnd to build tumor model and tumor significantly delays the healing of stoma.