摘要
作者报道了在盐酸－碘化钾－钒（ＩＶ）－乙醇－抗坏血酸体系中，用络合催化极谱法测定血中微量铅的方法。在此体系中，铅的峰电位为－０．５４Ｖ（ＶＳ．ＳＣＥ），铅浓度在０～１．００μｇ／３ｍｌ范围内与峰电流有良好的线性关系。样品加标回收率为７６．０％～１０５．０％，平均回收率为９１．０％。相对标准偏差为３．６％～７．０％。最低检测浓度为４５μｇ／Ｌ。采用本法对２４例正常人和６例铅作业工人血中铅的测定，结果与临床诊断符合。该法操作简便快速、灵敏度高、结果准确可靠，适合于正常人和铅作业工人血铅的测定。
A method for determination of trace lead in blood with complexcatlysis polarography was develop. The peak potential of lead is -0.54V in the system of hydrochloric acidpotassium iodide vanadium(IV) ethanol ascorbic acid. There is good linelar relationship between peak current and lead concentration in the range of 0 1.0μg/3ml. The recoveries of spiked samples ranged from 76.0% to 105.0% and the average recovery was 91.0% with RSD of 3.6% to 7.0%. The detection limit was 45μg/L. The blood samples of 24 normal people and 6 workers with occupational exposure to lead were measured and the results were concordant with the clinical diagnoses. The proposed method is simple, rapid, sensitive and reliable. It is suitable for determination of trace lead in blood.
出处
《华西医科大学学报》
CSCD
1996年第4期432-435,共4页
Journal of West China University of Medical Sciences
基金
中国预防医学科学院标准处资助
