应用MVR－PCR和毛细管电泳分析D1S8位点的结构多态性

ANALYSIS OF INTRA-CONSTRUCTIONAL POLYMORPHISM IN D1S8 LOCUS BY MVR-PCK AND CAPILLARY ELECTROPHORESIS

应用ＭＶＲ－ＰＣＲ扩增１０名无血缘关系个体的ＤＮＡ样本，扩增产物用毛细管电泳分离并用激光诱发荧光（ＬＩＦ）检测。结果显示每一个体可检出６～１４个扩增片段，个体间呈明显差异。该方法适合法医物证检验中分析微量或部分降解的ＤＮＡ样品。

NA samples from 10 unrelated individuals peripheral leucocytes were amplified by min-isatellite variable repeated PCR (MVR-PCR)PCR products were separated on capillary elec-trophoresis and detected by laser induced fluorescence(LIF).Each individual could be observed 6～14 peaks of DNA fragments on the chromatogram. The amplified fragments showed high poly-morphism.This approach is suitable fot analyzing trace and partial degraded DNA samples inforensic testing.