摘要
本研究在兔胸主动脉环模型上观察了二苯三硝基苯肼(DPPH)自由基对乙酰胆碱(ACh)诱导的血管内在依赖性舒张(EDR)反应的影响。结果显示:0.25μmol·L-1DPPH与血管环孵育10min,显著抑制了ACh诱导的血管EDR反应。此种抑制作用为可逆性。拉米普利和缓激肽能显著保护DPPH损伤的血管EDR反应。用DPPH建立自由基损伤模型的方法具有简单、方便、可靠、重复性好等优点。
he present paper describes for the first time a new simplemethod for damaging the en-dothelium cell(EC)-dependent relaxation by free radicals in vitro.The acetylcholine(ACh)-in-duced EC-dependent relaxation of rabbit aortic rings were injured in tissure bath by exposing for10 min to 0. 25μmol·L-1,1-Dipheny l-2-Picryl-Hydrazyl (DPPH), a stable free radicas. TheACh-induced relaxation was reduced from 89.3±1.6%to 36.1±3.2%immediately after l0minexposure to DPPH,and it gradually recovered to 43.3±2.1%and 54. 4±2.2%30 min and 60min respectively after washing out of DPPH, So the damage is reversible. The effecacy of thismodel was confirmed by the protective effect against the DPPH free radical damage provided by 5μmol·L -1 ramiprilat, an active form of ACE inhibitor ramipral or 1 μmol·L-1 bradykinine.This new method is simple, reliable and reproducible.
出处
《湖南医科大学学报》
CSCD
1996年第5期453-456,共4页
Bulletin of Hunan Medical University
关键词
二苯三硝基苯肼
自由基损伤
拉米普利
血管舒张
DPPH
free radicals
endothelium-dependent relaxation
ramiprilat
bradykinin,aortic rings