摘要
本文设计了两对引物，分别采用ＰＣＲ扩增结核杆菌异烟肼耐药性相关基因—ＫａｔＧ基因酶活性中心区，并用单链构象多态性分析（ＳＳＣＰ）及ＤＮＡ序列分析方法对临床分离的９株异烟肼敏感株、１１株异烟肼中、低度耐药株进行了耐药性分子机制的研究，结果发现中国人结核杆菌产生异烟肼耐药性的主要原因是由于ｋａｔＧ基因发生碱基点突变所致，本资料亦为快速检测结核杆菌异烟肼耐药性研究方法提供资料。
wo pairs of primers were designed.Nine isolates of isoniazid-sensitive and 11 isolates ofisoniazid-resistant M.tuberculosis were analyzed by polymerase chain reaction amplification of396bp sequence of katG gene.With the use of single-strand conformation polymorphism analysisand DNA sequencing,8 isolates in 396bp products and 5 isolates in 2l6bp products of the ll iso-lates showed point mutations and no deletion or insertion was detected.Thus,in China,pointmutations in katG gene can mainly account for inactivation of catalase-peroxidase,leading to iso-niazid resistance.The results provide laboratory evidence for detecting the isoniazid resistance in M.tuberculosis and also explore its molecular mechanism.
出处
《湖南医科大学学报》
CSCD
1996年第6期501-504,共4页
Bulletin of Hunan Medical University
基金
卫生部科研基金
关键词
结核杆菌
抗药性
聚合酶链反应
异烟肼
mycobacterium tuberculosis
drug resistance
polymerase chain reaction
SSCP
gene,catalase-peroxidase
