人卵巢癌p53基因5～8外显子的突变及其临床意义

Mutationsofp53GeneExons5to8inHumanOvarianCancerandItsClinicalSignificance

目的：检测人卵巢癌ｐ５３基因突变并探讨其与临床病理特征的关系。方法：用非同位素的聚合酶链反应方法－单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术，检测４１例原发性卵巢癌ｐ５３基因外显子５～８的突变。结果：检出突变１７例（４１．５％）；突变与患者年龄、病理分期、组织学类型无关，与分化程度、淋巴结转移有关。结论：卵巢癌ｐ５３基因突变率较高。ｐ５３基因突变为卵巢癌发生过程中的早期事件，可作为早期诊断和判断预后的检测指标之一。

Objectives:Todetectp53mutationsinhumanovariancancerandtolookforcorrela-tionsbetweenp53mutationandclinicopathologicfeatures.Methods:Mutationsinexons5～8ofp53genein41patientswithprimaryovariancancerwerescreenedbypolymerasechainreaction-singlestrandconformationpolymorphismanalysiswithnon-isotopicsilverstainingmethod.Results:Muta-tionsofp53werefoundin17cases(41.5%);p53mutationwasnotcorrelatedwiththeage,theFI-GOstageandthehistologictypeofthepatients.However,itwasassociatedwithgradeandlymphnodemetastasisinaunivariateanalysis.Conclusion:Mutationsofp53occurcommonlyinhumano-variancancer.p53mutationisanearlyeventinovariancarcinogenesisandmayserveasageneticmarkerforearlydiagnosisandprognosis.