氯化镓与α－干扰素对MGc80－3人胃癌细胞的协同抑制作用

SYNERGISTIC INHIBITION OF HUMAN GASTRIC CARCINOMA MGc80-3 CELL GROWTH BY GALLIUM CHLORIDE AND RECOMBINANT HUMAN α-INTERFERON

以人胃癌细胞株为实验对象，采用ＭＴＴ显色法在体外观察了金属类抗癌药物氯化镓与人α－干扰素的协同抑制作用。结果显示：单独加入α－干扰素无明显抑制作用，而与氯化镓同时加入则有明显的抑制作用，尤其是当先用α－干扰素或氯化镓孵育７２ｈ后，再交换加药其协同抑制作用更大。提示：在应用氯化镓治疗胃癌病人时可同时合用干扰素治疗，或先用α－干扰素一段时间再用氯化镓治疗其疗效可能更好。

In this study, we, using gastric carcinoma cell culture and MTT assay technique, observed the growth inhibition effect of gallium chloride (GC)and recombinant human α-interferon (α-INF) On poorly differenciated mucoid adenocarcinoma of human stomach MCc80-3 cell line. The results showed α-INF as single agents had only minimal antiproliferative effect. However, recombination of both drugs had synergistic inhibition of cell growth, causing cell death. Specifically, the cells were first incubated with either gallium or α-INF for 72 h and then reincubated with the alternate drug. It can markedly make cyostatia and kill cell. The results suggest that while GC as antitumor drug is taken by the pateints with cancer, we can treat them curative effect may be better.with α-INF.