海水养殖区甲胺磷降解菌的分离筛选及降解特性的研究

Studies on isolation and characteristics of methamidopho-degrading marine microorganism

目的分离、筛选降解海水养殖区甲胺磷的降解菌及确定最适的降解条件。方法从被有机磷污染的海水样中分离,以有机磷为唯一碳源反复驯化,分离筛选出1株高效降解甲胺磷的菌株M-1,并对其降解能力和所需条件进行测试。结果初步鉴定菌株M-1属于蜡样芽胞杆菌,菌株M-1最适生长温度和pH分别为25℃和8.0。Zn2+(200 mg/L),Cd2+(50 mg/L)与Pb2+(200 mg/L)不影响菌株M-1对甲胺磷的降解作用,但Cu2+(50mg/L),Cr2+(50 mg/L)对菌株M-1有毒性作用。结论海洋微生物在甲胺磷污染的海水养殖区自净中起着重要作用。

Objective To find organophosphate pesticide-degrading strain and its degradation conditions in marine contamination with organophosphate. Methods Isolated strain from marine environment sample by repeated subculture in liquid medium with methamidopho as carbon source. Degradability and one optimum organophosphatedegradation of bacterium was tested. Results The strain M-1 was identified as Bacillus cereus. The optimum temperature and pH value were 37 ℃ and 8.0 respectively. Zn^2＋ （200 mg/L） ,Cd^2＋ （50 mg/L） and Pb^2＋ （200 mg/L） did not inhibit growth and biodegradation of organophosphate of the strain, but the inhibitory effect was found on Cu^2＋ （50 mg/L） and Cr^2＋ （50 mg/L）. Conclusions These findings indicated that marine microorganism might be useful for bioremediation of marine contaminated with organophosphate.