川芎嗪逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性

Reversal of the resistance to adriamycin in human breast cancer cell line MCF-7/ADM by TMP

目的探讨川芎嗪(tetramethylpyrazine,TMP)逆转人乳腺癌MCF-7/ADM细胞对阿霉素(ADM)的耐药性。方法MTT法测定细胞的药敏性,荧光分光光度法检测细胞内阿霉素浓度的变化,流式细胞术检测耐药细胞凋亡百分率的变化。结果非细胞毒性剂量(320 mg/L)及低毒剂量(1250 mg/L)川芎嗪均能显著降低MCF-7/ADM的IC50(P<0.01),逆转倍数分别为2.13倍和2.82倍;均能显著增加耐药细胞内ADM的浓度(P<0.01)。320 mg/L川芎嗪能显著增加耐药细胞的凋亡百分率(P<0.01)。结论川芎嗪具有部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,其逆转机制与增加细胞内ADM浓度有关。

Objective To study the reversal or the resistance to adriamyein in human breast cancer cell line MCF-7/ADM by TMP. Methods The drug sensitivity of MCF-7/ADM cells was measured by MTT assay. The intracellular concentration of ADM was determined by fluorescent-spectrophotometry. The cell apoptosis percentage of MCF-7/ADM was demonstrated by flow cytometry. Results The IC50 value of TMP in MCF-7/ADM cell line was significantly decreased by the non-cytotoxic dose（320 mg/L） or low-cytotoxic dose（1250 mg/L） of TMP（P〈.0. 01） and the resistance-reversing effect was 2.13-folds, 2.82-folds respectively compared with the IC50 value of ADM in MCF-7/ADM cells. The intracellular ADM concentration in MCF-7/ADM cells were significantly elevated by TMP at the same dose（P〈0.01）. The cell apoptosis percentage in MCF-7/ADM cells was significantly increased by TMP （P〈0.01）. Conelusion TMP could partially reverse the resistance to ADM in human breast cancer MCF-7/ADM cell line,which may be related to the increased intracellular ADM concentration.