利用16S rRNA基因同源性分析鉴定两株明串珠菌

Using cluster analysis of 16S rRNA gene to identify two Leuconostoc species

从酸马奶中分离出2株明串珠菌KLDS 5.0301和KLDS 5.0302,对2株菌的16S rRNA基因经PCR扩增测序,将测序结果同该属内菌株的16S rRNA序列作多序列比较,并建立明串珠菌属的系统发育树。结果表明,KLDS 5.0301的16S rRNA序列同L.garlicum的同源性百分比为100%。KLDS 5.0302的16S rRNA序列同L.mesenteroidesLM2菌株的16S rRNA序列的同源性百分比为99.9%。根据系统发育树的结果,将KLDS5.0301鉴定为L.garlicum,KLDS 5.0302鉴定为L.mesenteroides。菌株KLDS 5.0301和KLDS 5.0302的16SrRNA序列已经在GeneBank申请国际序列注册号,分别为DQ239691和DQ297412。

To further differentiate the two strains of Leuconostoc KLDS 5. 0301 and KLDS 5. 0302 isolated from homemade koumiss, the 16S rRNA gene of them were amplified in vitro and sequenced. Then a phylogenetic tree was constructed by multiple alignments of these two sequences with other 16S rRNA sequences of Leuonostoc. The results indicated that the homology of KLDS 5. 0301 with L. garlicum was 100%, and the homology of KLDS 5. 0302 with L. mesenteroides LM2 was 99. 9 %. According to the results of phylogenetic tree, KLDS 5. 030 was classified as L. garlicum,and KLDS 5. 0302 was classified as L. mesenteroides.