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HPLC-UV与HPLC-ELSD测定环丙沙星含量之比较 被引量:3

The comparison of determination of ciprofloxacin by HPLC-UV and HPLC-ELSD
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摘要 目的:探讨利用HPLC-紫外检测器(UV)和HPLC-蒸发光散射检测器(Evapo-rative light scattering detector,ELSD)测定乳酸环丙沙星氯化钠注射液中环丙沙星含量,比较两种检测方法的优劣。方法:色谱条件为:色谱柱:Kromasil C-18,5,μ250×4.6mm;流动相:三氟乙酸∶乙腈=80∶20(V/V);流速:1mL/m in;柱温:25℃;检测波长:277nm;蒸发光散射检测器条件:雾化温度30℃,漂移管温度65℃。结果:环丙沙星注射液中的各种成分在上述色谱条件下能完全分离,分析时间仅需20m in;两种测定方法结果一致。结论:紫外检测器更加灵敏和稳定,而蒸发光散射检测器则适用于更多的物质。 Objective: To approach an assay of determination of ciprofloxacin in Ciprofloxacin Lactatc and Sodium Chloride Injection by HPLC - UV and HPLC - ELSD methods. Make a comparison between the two different methods. Methods: The separation was performed on a Kromasil C - 18 column (5 μ,250 × 4.6mm). The; n,ohile phase consists of trifluoroacetie acid(0. 1% ) -acetonitrile(80: 20) at a flow rate of 1.0 mL/min. Determination wavelength: 277mn. An evaporative light -scattering detector (ELSD) Modcl 200(SoftA, USA) was used, its para,neters were set as follows: spray temperature at 30℃, drift tube temperature at 65℃. Results: Good separation of Ciprofloxacin and related substances can be achieved respectively under the two chromatographic system only in 20 minutes. The two methods could give the same determination. Conclusion: The UV detection is more sensitive and more steady, and ELSD detection could be used in determination of more substances.
出处 《现代仪器》 CAS 2006年第6期29-31,共3页 Modern Instruments
关键词 高效液相色谱法 紫外检测器 蒸发光散射检测器 环丙沙星 HPLC UV ELSD Ciprofloxacin Content determination
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