艾康疟原虫检测试剂盒现场应用效果观察

Observation on the results of ACON Malaria Kit in diagnosis of Plasmodium falciparum and Plasmodium vivax in field.

目的评价国产艾康疟原虫(pf/pv)检测试剂盒现场应用效果。方法以镜检方法作为金标准比较艾康疟原虫检测试剂盒现场应用效果。结果336例样本均用镜检方法和艾康疟原虫试剂盒诊断,镜检阳性155例,阴性181例;试剂盒诊断阳性156例,阴性180例,试剂盒诊断疟疾的灵敏度为99.4%,特异度为98.9%。试剂盒诊断间日疟的灵敏度为98.8%、特异度为100.0%;试剂盒诊断恶性疟的灵敏度为100.0%、特异性为99.3%。未发现交叉反应。结论艾康试剂盒诊断疟原虫敏感性与特异性均高,且一次能测出单纯恶性疟或单纯间日疟的感染,操作简单,结果显示直观、快速,在常温下保存稳定,适合疟疾流行区的基层单位使用。

Objective To evaluate the efficacy of ACON Malaria pf/pv Kit in detecting P. Falciparum and P. Vivax in field. Methods Blood examination by microscopy was taken as gold standard and the efficacy of ACON Kit in detection of Plasmodium falciparum and Plasmodium vivax in field was evaluated, Results A total of 336 cases were detected both by microscopy and A-CON kit. There 155 were positive and 181 were negative by microscopy, 156 were positive and 180 were negative by ACON kit with a sensitivity and specificity of 99,4 % and 98,9 %. The sensitivity and specificity of ACON Kit in detection of P. Vivax were 98.8 % and 100% respectively, The sensitivity and specificity of ACON Kit in detection of P. Falciparum were 100% and 99,3% respectively. There no cross reaction was observed. Conclusion The ACON Kit is both highly sensitive and specific, it is stable when kept under morn temperature. In addditon, it is capable of detection of single P. Vivax and P. Falciparum infection simultaneously in a rapid simple and directly way suitable for application in field of malaria epidemic areas.