泌尿生殖道支原体检测及药敏结果分析

Detection of urogenital mycoplasma and analysis of results of drug sensitivity test.

目的了解疑似非淋菌性尿道炎（NGU）患者解脲支原体（UU）和人型支原体（Mh）的感染情况和耐药性。方法采用珠海益民生物工程制品厂提供的UU和Mh试剂盒进行支原体培养、鉴定和药敏试验。结果798份被检标本中，检出支原体阳性275份，总检出率34．5％，男性检出率19.3％，女性检出率45.3％，女性明显高于男性（P〈0．001）；UU、Mh、UU＋Mh检出率均以女性高于男性。单纯UU感染以交沙霉素、强力霉素、罗红霉素、克拉霉素和美满霉素的耐药性较低（1．8％，14．3％）；Mh感染以交沙霉素、强力霉素和美满霉素的耐药性较低，为11.1％；对UU＋Mh混合感染耐药性较高，仅交沙霉素的敏感率较高，为82．2％。结论对NGU患者应作支原体培养和药敏试验，动态监测其耐药性，指导临床医生合理用药，以减少耐药菌株的产生。

Objective To understand the infection rates of Ureaptasma urealyticum （Uu） and Mycoplasma hominis （Mh） and analyze the status of drug resistance of mycoplasma in nongonococcal urethritis（NGU） patients Methods Uu and Mh were cultured and mycoplasma isolates were identified and drug sensitivity test was conducted with Uu and Mh test kit provided by Yeoman Biological Engineering Factory in Zhuhaihe, Results A total of 275 cases were tested positive for mycoplasma out of the 798 specimens with a positive rate of 34.5%, Being 19.3% in the males and 45.3% in the females, showing significant difference （P 〈 0.01）, The resistance rate of Uu to josamycin, doxycyclinc, roxithromycin, clarithromycin and minocycline were low （ 1.8 % - 14.3 % ）, while that oi Mh to josamycin, doxycycline and minocycline were low （11.1% ）. The resistance of mixed infection with Uu and Mh were high. Josamycin was the most effective antibiotics for Un and Mh（82.2 % ）, Conclusion Un and Mh be cultured and drag sensitivity test be carried out in order to guide clinical use of antibiotics and delay the development of drug resistance.