苹果废渣中天然抗氧化物的提取、分离及活性研究

Study on the extraction,isolation and activity of natural antioxidant from apple pomace

以苹果渣为原料,用不同浓度乙醇提取得到苹果多酚粗提物,再分别用乙酸乙酯萃取和薄层层析纯化,用猪油POV值测试为跟踪方法对各组分进行筛选,然后以BHT为对照,对最高活性组分与V_c、PG、柠檬酸的协同作用进行了系统探讨。结果表明,95％乙醇的提取效果最好,其干物质以无水乙醇重溶后活性显著提高；PG与苹果多酚的协同效果最好,是对照BHT的2．33倍。

In this paper apple polyphenols were obtained from apple pomace by extraction with various ethanol-water solvent systems and then extracted by ethyl acetate and purified through silica gel thin -layer chromatography respectively, The antioxidant activity was monitored with lard POV value assay. The synergism between the highest active component and Vc, PG or citric acid were systematically investigated, and the result showed that the best extraction solvent was 95% ethanol, after its drying and re-dissolving with ethanol the activity was even better, the synergism between apple polyphenols and Vc was the strongest in all samples tested.