天麻多糖水提取工艺优化研究

Optimization of aqueous extraction of Gastrodia elata blume polysaccharides

研究了天麻多糖提取工艺中提取时间、提取温度、料水比对天麻多糖提取率的影响，并对这3个工艺参数进行了优化。结果表明，提取温度、提取时间和料水比对天麻多糖提取率的影响程度都达到高度显著水平。SAS软件处理数据得二次回归方程为：Y=9273＋0.488X1＋0.308X2＋0.185X3-0.495X1^2-0373X2^2-0211X2^2＋0．004X1X2-0．024X1X3-0．016X2X3。响应曲面分析结果表明，水法提取天麻多糖的最佳工艺条件为：提取温度为58℃，提取时间36min．料水比1：37．

In this paper, the effects of extraction time, temperature, and ratio of Gastrodia elata BI. to water （W/V） on the yield of Gastrodia elata BL polysaccharides were researched and the parameters for optimum yield were obtained by using SAS software. The results showed that temperature, time and ratio of Gastrodia elata BI. to water had significant effects on the yield of Gastrodia elata BI. polysaccharides, and the regression equation was Y =9.273 ＋0.488X1 ＋ 0.308X2 ＋0.185X3 -0.495X1^2 -0.373X2^2 -0.211X3^2 ＋ 0.004X1X2 -0.024X1X3 -0.016X2X3,Canonical analysis showed that the optimum parameters were： extraction for 36min at 58℃ with 1：37 ratio of Gastrodia elata BI, to water.