高压液相色谱法测定番茄果实中的α-，β-胡萝卜素和番茄红素

The determination of lycopene,α-carotene,β-carotene in hard ripening stage tomato by HPLC

使用丙酮提取番茄果实中的色素,用反相高效液相色谱测定坚熟期番茄中的α-胡萝卜素、β-胡萝卜素和番茄红素。流动相梯度A为乙腈和水(97：3,v/v),流动相梯度B为乙酸乙酯,流动相B在80min内由0上升到100％,流速为0．5mL/min,500nm检测波长下进行检测,α-胡萝卜素、β-胡萝卜素和番茄红素的回收率依次为：90．87％～92．60％,90．22％～92．75％和91．08～93．48％。坚熟期番茄果实中检测到α-胡萝卜素、β-胡萝卜素和番茄红素的含量分别为3．718、78．48和34．92mg/kg。

A reversed -phase high performance liquid chromatographic method was used to determine carotenoids extracted with acetone from hard ripening stage tomato fruit. Mobile phase A consisted of acetonitrile and water （97：3,v/v） and mobile phase B was ethyl acetate. Binary gradient was performed linearly with the original of 100% A and finished in 80 minutes with 100% B. Carotenoids were detected at 500nm. It was shown that recoveries of α-carotene,β-carotene and lycopene were 90,87%-92.60%,90.22%-92.75% and 91.08%-93.48% o The hard ripening stage tomato contained α-carotene （3.718mg/kg）, β-carotene （78.48mg/kg） and Iycooene（34.92mg/kg）.