双探针实时荧光定量检测HCV RNA的研究

Study on Real-Time Fluorescence Quantificative Determination of HCV RNA by using Double Probe

目的建立双探针荧光定量HCV RNA检测方法,探讨解决HCV RNA定量灵敏度和特异性问题。方法对89例抗HCV抗体阳性和220例阴性样本用双探针荧光定量法和两种商品荧光定量试剂方法进行检测。结果双探针荧光定量法阳性率分别为91.0%(81例)和2.72%(6例)。两种商品荧光定量试剂方法阳性率分别为82.0%(73例)和0.9%(2例);79.7%(71例)和2.27%(5例)。结论双探针荧光定量提高了HCV RNA检测的灵敏度和特异性。

Objective To establish real-time fluorescence quantitative reverse transcription-polymerase chain reaction（RFQ-RT-PCR） for HCV RNA by using double-probe ,and to resolve the problems of sensitivity and specificity of HCV RNA quantification. Method A total of 89 anti-HCV positive cases and 220 anti-HCV negative cases were quantified with RFQ-RT-PCR by using double-probe, and were also detected by other two HCV RNA quantification kits simultaneously. Results HCV RNA positive rate was 91.0% （81 cases） and 2. 72% （6 cases） respectively in RFQ-RT-PCR by using double-probe,while it was 82.0% （73 cases） and 0. 9% （2 cases） ,79.7% （71 cases） and 2.27% （5 cases） by using other two kits. Conclusions RFQ-RT-PCR assay using double-probe increases the sensitivity and specificity of HCV RNA detection.