驱虫斑鸠菊抗肿瘤活性部位的初步研究

Studies on Anti-tumor Activity of Extracts of Vernonia anthelmintica willd

目的:对维吾尔族民间药材驱虫斑鸠菊Vernonia anthelmintica willd.果实抗肿瘤活性部位进行初步研究。方法:运用体外抗肿瘤生物活性跟踪的方法,采用人胃癌细胞(BGC-823)、人肝癌细胞(HepG-2)和黑色素瘤细胞株(B16、A375)对驱虫斑鸠菊氯仿、丙酮、乙醇、水提取部位进行体外抗肿瘤活性筛选,并利用高速逆流色谱和Sephadex LH-20柱层析对活性部位进行初步分离,通过HPLC对不同提取部位进行分析。结果:经体外活性筛选显示氯仿、丙酮、乙醇提取部位对肿瘤细胞增殖均具有一定的抑制作用;得到一个单体化合物,经结构分析为紫铆查尔酮;同时,通过对HPLC的色谱图分析显示各提取部位的色谱图具有一定的相似性。结论:本研究可能对天然植物有效部位或有效成分的研究提供了一种新的思路。

Objective： To analyse chemical components of volatile oil from leaves of Artemisia Borealis. Methods： The volatile oil was extracted from A. Borealis by steam distillation and analysed by GC- MS. Results： 52 peaks were separated from the essential oil ofA. Borealis, 48 of them were identified by GC-MS analysis, indicating aromadendrane-4-ol is the highest component in the essential oil, accounting for 24.73%. Conclusion： The result provided solid and scientific proof for the further exploitation and utilization of A. Borealis.