产抗菌蛋白芽孢杆菌R21-4的鉴定及其抗菌谱研究

Identification of Antagonistic Bacillus sp. R21-4 and Research of Its Antimicrobial Mechanism

筛选的芽孢杆菌R21-4对某些革兰氏阳性、革兰氏阴性细菌及真菌均有抑制作用,具有较广的抑菌谱,特别是对食品中的常见腐败菌,如蜡样芽孢杆菌、大肠杆菌、产气荚膜杆菌、产气肠杆菌、荧光假单胞菌等均有明显抗菌效果。在对其进行生理生化以及16Sr RNA基因序列测定和聚类分析软件的分析后,鉴定菌株R21-4为枯草芽孢杆菌Bacillus subtilissp.R21-4,并采用琼脂块法和生长曲线法对其抗菌机理进行了研究。

Bacillus sp. R21 - 4 demonstrated a broad spectrum of activity against some Gram-negative bacteria, Gram-positive bacteria, and some fungi. Especially have an antagonistic function to some spoilage of B. cereus, Escherichia coli, Clostridium perfringens, Enterobacter aerogenes, Pseudomonas fluorescens. The strain was identified Bacillus sp. R21 -4 after physiological-biochemical characteristic experiments, sequencing of 16Sr RNA and cluster analysis. The antimicrobial mechanism was processed by agar method and growth curves.