RP-HPLC同时测定保和丸中5种有机酸

Simultaneous determination of five organic acids in Baohewan by RP-HPLC

采用反相高效液相色谱法，在Lichrospher C18分析枉中，以（NH4）2HPO4-H3PO4水溶液（pH值为2．8）为流动相，建立了保和丸（浓缩丸）中草酸、酒石酸、苹果酸、柠檬酸、琥珀酸的含量测定方法．结果表明，在选定实验条件下，上述5种酸的线性范围分别为2．6×10^-7～1．3×10^-5g，3．0×10^-7～1．5×10^-5g，4．8×10^—7～2．4×10^-5g，6．9×10^-7～3．5×10^-5g，7．7×10^-7～3．9×10^-5g；检出限分别为6．4×10^-9g，7．5×10^-9g，1．2×10^-8g，1．8×10^-8g和2．0×10^-8g．该方法用于保和丸中草酸、酒石酸、苹果酸、柠檬酸、琥珀酸的定量测定，取得比较满意的结果．

This article reports a method for the simultaneous separation and determination of oxalic acid （OA）, tartaric acid（TA）, malic acid （MA）, citric acid（CA） and succinic acid（SA） in Baohewan by reversed-phase high-performance liquid chromatography （RP-HPLC）, Analytical column was Lichrospher C18, mobile phase was （NH4）2HPO4-H3PO4 aqueous solution（pH 2.8） and detected wavelength was 210 nm. The results show that the linear ranges for five constituents are 2, 6 × 10^-7-1.3×10^-5g,3.0×10^-7-1.5×10^- 5g,4.8×10^-7-2.4×10 ^-5g,6.9×10^-7-3.5×10^-5g,7.7× 10^- 7-3.9 × 10^-5g, respectively. The limitary detection for five constituents are 6.4 × 10^-9g,7.5 × 10^- 9g, 1.2 × 10^- 8g, 1.8 × 10^- 8g, 2.0 ×10^- 8g, respectively, The proposed method has been applied to the determination of OA,TA, MA,CA and SA in Baohewan with satisfactory results.