鸡血清与卵黄中抗中华眼镜蛇毒IgY动态变化研究

Research on the dynamic change of anti-Naja atra venom IgY in serum and yolk of hens

目的探索特异性IgY的产生和变化规律。方法用眼镜蛇毒原毒免疫产蛋母鸡,ELISA定期检测卵黄中的抗体效价变化,小鼠体外中和实验检测其生物活性。第1次免疫40周后,眼镜蛇毒攻击已免疫母鸡,检测攻击前后鸡血清中抗体效价变化情况,未经眼镜蛇毒免疫的母鸡作阴性对照。结果经免疫后第7天蛋黄中即可检测到抗体,经多次加强免疫,40周时蛋黄中还能保持高效价的抗体,通过分离纯化,此抗体可保护实验小鼠免受4 LD50眼镜蛇毒的攻击;同时,鸡血清中也保留着较高效价的抗体,可中和4 LD50以上的眼镜蛇毒。结论用眼镜蛇毒免疫鸡,经多次加强免疫,卵黄和鸡血清中可持久保持高效价的特异性抗体,初步检测此抗体可中和4 LD50的蛇毒。

Objective To explore the generating and variance regularity of antibody of anti- Naja atra venom through immunizing hens. Methods Use the original venom of Naja atra to immunize hens. The titer of IgY was measured by ELISA, challenge dose of venom pre-incubated with antibody was injected into mice to test the bioactivity of IgY. 40 weeks after first immunization,Naja atra venom was used to attack the immunized hens, and then the titer vary of antibody was tested in serum. Take the un-immunized hens as control. Results IgY of anti-Naja atra venom ould be detected on the 7th day after the first immunization, and maintained in high titer for over 40 weeks after the several boosters. The purified antibody from yolks could completely protect mice from the attack of 4 LD50 of Naja atra venom, and serum maintained high titer of antibody which could also protect the hens from the attack of 4 LD50venom. Conclusion Immunized by the original cobra venom,high titer and special antibody can be generated in the serum and yolk of hens, which can neutralize 4 LD50 of Naia atra venom.