两检测系统测定血清电解质的方法学对比与偏差评估

Comparative Evaluation of the Serum Electrolyte Determination with Two Different Analysis Systems

目的分析对比美国强生Vitros-350全自动生化分析仪度HITACHI7170型全自动生化分析仪两检测系统测定血清电解质（K^＋、Na^＋、Cl^-）结果，并作偏差评估。方法按照美国临床实验室标准化委员会EP9-A文件的要求，每天选取8份患者样本分别用两种方法按1～8，8～1的顺序进行血清电解质（K^＋、Na^＋、Cl^-）测定，共测定5d，记录检验结果，去除离群点，对其进行方法间偏差评估。结果两方法K^＋、Na^＋、Cl^-测r为0．996-0．999，在不同医学决定水平处的预期偏差均未超过CLIA＇88允许误差的一半，相关良好。结论强生Vitros-350及HITACHI7170两检测系统测定血清电解质（K^＋、Na^＋、Cl^-）结果基本一致，临床检测过程中可任选一种进行电解质测定。

Objective To evaluate the results of electrolyte assay by using two automatic biochemistry analyzer systems.. Johnson Vitros-350 and Hitachi 7170. Methods According to the protocol described by the NCCLS document EP9-A, 8 clinical samples were taken each day and the serum contents of K^＋ ,Na^＋ and Cl^- were measured by using the two analysis systems according to the sample sequence 1 to 8 and 8 to 1. In five days the results of 40 case samples were recorded and the deflective data were eliminated,the deflection was also estimate. Results There was a better correlation between the two methods of electrolyte assay and the correlation modules （r） was 0. 996-0. 999. In the different points of medical decision standard,the expectant deflection did not exceed the half of allowable error of CLIA＇88. Conclusion The assay resuits of serum electrolytes（K^＋ ,Na^＋ ,Cl^-）by using Johnson Vitros-350 system are basically consistent with those by using Hitachi 7170 system.