血液透析液浓缩B液细菌污染控制方法的探讨

The Strategies for Prevention of Bacterial Contamination in Concentrated Hemodialysis Fluid B

目的调查血液透析液浓缩B液细菌数超标的原因,找出控制方法。方法检测出入口透析液、透析用水、A、B液,配置B液的容器及溶质的细菌菌落数;以查出原因前的监测资料为对照组,采取措施后的监测资料为试验组。结果B液平均含菌量1052±122cfu/ml,配置B液的容器含菌量845±318cfu/ml,严重污染。其余的均不超标。对照组B液的平均含菌量968±515cfu/ml,合格率21%;试验组146±32cfu/ml,合格率100%。两组资料经统计学处理t=11·918,P<0·001,χ2=67·16,P<0·005,差异有显著性。结论浓缩B液污染的主要原因为配置容器污染,未加盖密封保存。采取措施后B液含菌量明显减少,合格率明显提高,达100%。并对B液的细菌学监测标准进行了探讨,认为采用≤200cfu/ml的标准比较恰当。

Objective To evaluate the cauls of unqualified level of bacterial in concentrated dialysis fluid B, and find the strategies for the prevention of contamination. Methods The levels of bacterial contamination were detected. The datum were divided into the experimental group and control group . In former there was no prevention method of contamination. Results The average bacteria colony forming unit per milliliter in concentrated dialysis fluid B, in the container putting dialysis fluid B was 1052 ± 122, 845 ±318 cfu/ml, respectively. The contaminations were very serious. In the experimental group, the average bacteria colony forming unit per milliliter in concentrated dialysis fluid B was 968 ± 515 cfu/ml, the rate of qualified was 21% , in the control group , 146± 32 cfu/ml, 100% , respectively. There was remarkable significance between the two groups. Conclusion The major causes of concentrated dialysis fluid B polluting was contamination of the container which contains dialysis fluid B , and no cover for preserving. It is suitable that standard of bacteria contamina- tion is ≤200 cfu/ml.