冰冻切片放射自显影技术在微剂量研究中的应用

Application of study in microdosimetry with the frozen sectioning microautoradiogtraphy technique

目的用放射自显影技术观察放射性核素在细胞水平的分布。方法采用放射自显影和冰冻切片技术,建立银颗粒密度与放射性药物强度的刻度曲线．确定放射性药物^(131)I和(99m)Tc的微观分布,基于核素的微观分布数据,建立微剂量的剂量估算模式。结果银颗粒密度与施入放射性药物比活度的相关系数分别为0．9915和0．9963,刻度系数分别为1．59×10^(-4)Bq和6．48×10^(-5)Bq。结论^(131)I和^(99m)Tc在细胞水平的分布是不均匀的,银颗粒多数分布在细胞质中,因此在计算细胞水平的剂量时应考虑到其分布的不均匀性。

Objective To survey the distribution of radionuclide in cell level by frozen sectioning microautoradiogtraphy technique. Methods The article will describe the micro distribution of ^131 I and ^99m Tc using autoradiogtraphy and frozen sectioning technique. Results To describe the relationship between argent grain density and nuclide radioactivity a regression curve of ^99m Tc and ^131 I was depicted and the R value calculated to be 0.9915 and 0.9963 respectively, The scale coeflqcient of argent grain were 1,59 × 10^-4Bq and 6.48 × 10^-5 Bq respectively. Conclusion The distribution of radiomedicament ^131 I and ^99m Tc at cellular level is nonunidoem.