摘要
目的:用高效液相色谱法测定石韦中木犀草素的含量。方法:采用Shimadzu C_(18)柱(150mm×4.6mm,5μm),流动相为甲醇-0.2%H_3PO_4溶液(50:50),流速1.0mL/min,检测波长254nm,柱温室温,用外标法定量,测定石韦中木犀草素的含量。结果:木犀草素的线性范围0.0325~0.391μg,r=0.9999,回收率95.85%,RSD为0.27%。结论:该方法简便,快速,线性关系良好,适合作为石韦中木犀草素含量测定的方法。
Objective:To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods :The HPLC method was used to determine the content of luteolin from Pyrrosia lingua. The analysis was carried out on Shimadzu C18 column( 150mm×4.6mm,5μm). The mobile phase was MeOH- 0.2% H3PO4 (50 : 50). Flow-rate was 1.0mL/min. Wave-length was 254nm. Temperature was room temperature, Results: The method was simple and had a good linear relationslfip. The liner range of luteolin is 0. 40 -2. 00μg and gave a correlation (r) of 0. 999 6. The recovery was 95.85% ,RSD 0. 27%. Conclusion: The method was easy to determine the content of luteolin from Pyrrosia lingua ,
出处
《药学实践杂志》
CAS
2006年第6期359-361,共3页
Journal of Pharmaceutical Practice