HPLC法测定石韦中木犀草素的含量

Quantitative determination of luteolin in Pyrrosia lingua by HPLC

目的：用高效液相色谱法测定石韦中木犀草素的含量。方法：采用Shimadzu C_(18)柱(150mm×4．6mm,5μm),流动相为甲醇-0．2%H_3PO_4溶液(50：50),流速1．0mL/min,检测波长254nm,柱温室温,用外标法定量,测定石韦中木犀草素的含量。结果：木犀草素的线性范围0．0325～0．391μg,r=0．9999,回收率95．85%,RSD为0．27%。结论：该方法简便,快速,线性关系良好,适合作为石韦中木犀草素含量测定的方法。

Objective：To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods ：The HPLC method was used to determine the content of luteolin from Pyrrosia lingua. The analysis was carried out on Shimadzu C18 column（ 150mm×4.6mm,5μm）. The mobile phase was MeOH- 0.2% H3PO4 （50 ： 50）. Flow-rate was 1.0mL/min. Wave-length was 254nm. Temperature was room temperature, Results： The method was simple and had a good linear relationslfip. The liner range of luteolin is 0. 40 -2. 00μg and gave a correlation （r） of 0. 999 6. The recovery was 95.85% ,RSD 0. 27%. Conclusion： The method was easy to determine the content of luteolin from Pyrrosia lingua ,