内分泌源性血管内皮生长因子在胃癌中的表达

Expression of endocrine glands-derived-vascular endothelial growth factor in human gastric cancer tissue

背景与目的:内分泌源性血管内皮生长因子(endocrine glands-derived-vascu lar endothelial growth fac-tor,EG-VEGF)的信号传导途径影响的肿瘤血管形成。本研究检测胃癌组织中EG-VEGF的表达状态,探讨胃癌组织中EG-VEGF的表达与肿瘤的临床病理因素之间的关系。方法:收集2003年1月—2005年12月我院普外科胃癌根治术病例106例,采用免疫组化染色法及IMS图像分析系统检测胃癌组织、癌旁组织及正常胃组织中EG-VEGF的表达情况,结合临床病理资料回顾性分析EG-VEGF与各临床病理因素之间的关系。结果:①EG-VEGF的表达在正常胃粘膜组织和癌旁组织中标记指数(labeling index,LI)分别为24.3±10.4、31.4±12.2,差异无显著性。胃癌组织中EG-VEGF抗原表达的标记指数41.6±13.3,较正常胃组织和癌旁组织显著升高(P<0.01)。②EG-VEGF标记指数与胃癌临床分期相关(P<0.05);EG-VEGF标记指数与肿瘤的浸润深度、淋巴结转移状态、远处转移情况、TNM分期及肿瘤分化程度密切相关(P<0.01)。结论:①胃癌组织中EG-VEGF的标记指数明显高于正常胃组织和胃癌癌旁组织。②胃癌组织中EG-VEGF的表达与肿瘤分化程度、浸润深度、淋巴结转移状态、远处转移状况、TNM分期有关。

Background and purpose： Endocrine glands-derived-vascular endothelial growth factor（EG-VEGF） influences angiogenesis of tumor. We investigated the expression of EG-VEGF in human Gastric cancer tissue. Methods： 106 patients with gastric cancer who received radical resection from 2003 to 2005 in Shanghai Sixth People＇ s Hospital were enrolled into the study and EG-VEGF status were evaluated by immunohistochemical assay with their paraffin slices, the in- tensity of EG-VEGF was quantitated by IMS figure analysis system and EG-VEGF positive index is recorded. All data were collected in the format as mean ＋ standard deviation and analyzed with SAS6.12. Results： ① The positive index of EG- VEGF were 31.4 ± 12.2 in tissue near gastric cancer and 24.3 ± 10.4 in normal gastric tissue（ P 〉 0.05）. The expression of EG-VEGF in the gastric cancer tissue was 41.6 ± 13.3, which significantly higher than that in tissue near gastric cancer and normal gastric tissue（ P 〈 0.01 ） ; ② The positive index of EG-VEGF was correlated with clinical classification. The higher positive index of EG-VEGF was, the more depth of gastric wall penetration, lymph nodes involvement, distal metastasis, and the differentiation of tumor（ P 〈 0.01 ）.. Conclusions： ① The positive index of EG-VEGF is highest in gastric cancer comparing to tissue near gastric cancer and normal gastric tissue. ② The expression of EG-VEGF is related to the cancer radius, clinical classification, the depth of gastric wall penetration（ T）, lymph nodes involvement（ N）, distal metastasis（M） and cancer differentiation.