外周注射福尔马林诱导大鼠前扣带皮层中ERK-CREB的激活

THE ACTIVATION OF EXTRACELLULAR SIGNAL-REGULATED KINASE AND cAMP RESPONSIVE ELEMENT BINDING PROTEIN IN THE ANTERIOR CINGULTE CORTEX INDUCED BY PERIPHERAL INJECTION OF FORMALIN

目的研究外周福尔马林刺激诱导的大鼠前扣带皮层中细胞外信号调节激酶(ERK)和cAMP反应元件结合蛋白(CREB)的激活。方法大鼠单侧足底皮下注射5%的福尔马林,在刺激后的不同时间点将大鼠进行灌注固定或直接取前扣带皮层组织,用免疫组织化学和Western blotting方法观察前扣带皮层中磷酸化ERK和磷酸化CREB的激活情况。结果单侧足底皮下注射福尔马林能诱导双侧前扣带皮层中ERK和CREB的磷酸化。磷酸化的ERK和磷酸化CREB的表达高峰分别在刺激后的3min和30min。非磷酸化的CREB和ERK没有显著变化。结论大鼠前扣带皮层能够接受外周伤害性信息的传入,激活的ERK和CREB可能与疼痛的原发性不愉快有关。

Objective To investigate the activation of extracellular signal-regulated kinase （ERK） and cAMP-responsive element binding protein （CREB） in the anterior cingulate cortex （ACC） induced by peripheral injection of formalin. Methods 5 % formalin was injected subcutaneously into the unilateral hindpaw. The rats were perfused or killed and the ACC dissected at different time points. The activation of phosphorylated ERK （pERK） and phosphorylated CREB （pCREB） in the ACC were observed by immunohistochemical and Western blotting techniques. Results Subcutaneous injection of formalin induced the activation of pCREB and pERK in the ACC. Formalin-induced the activation of pERK and pCREB in the ACC peaked at 3 min and 30 min after stimulation, respectively. The total ERK and CREB had no significant changes. Conclusion The ACC could receive peripheral noxious information and the ERK and CREB in the ACC might involve in the processing of the primary unpleasentness of pain.