变应性鼻炎大鼠鼻黏膜补体C3和C4 mRNA的表达

mRNA expression of complement C3 and C4 in rat nasal mucosa with allergic rhinitis

目的探讨大鼠鼻黏膜补体成分C3、C4mRNA表达水平及其与变应性鼻炎的关系。方法健康SD大鼠20只,随机分为实验组和对照组,每组各10只。实验组以0·1%卵清蛋白辅以氢氧化铝佐剂建立大鼠变应性鼻炎模型,采用逆转录-聚合酶链反应(reversetranscriptase-polymerasechainreaction,RT-PCR)方法对实验组和对照组大鼠鼻黏膜补体C3、C4mRNA表达水平进行定量研究。结果实验组及对照组鼻黏膜中均有C3、C4mRNA表达。C3mRNA基因表达的相对强度实验组为6183±1376(x-±s,下同),对照组为4444±989;C4mRNA基因表达的相对强度实验组为4398±948,对照组2771±407。实验组C3、C4mRNA表达水平均有明显上调,差异均有统计学意义(t值分别为3·347、2·385,P值均<0·05)。结论变应性鼻炎鼻黏膜组织中补体C3、C4mRNA表达水平上调,提示补体系统参与变应性鼻炎的病理生理过程。其高表达可能与补体经典激活途径的部分激联反应有关。

Objective To investigate the level of mRNA expression of complement C3 and C4 in rat nasal mucosa and to reveal the relationship with the pathogenesis of allergic rhinitis （ AR ） . Methods Twenty healthy SD rats were randomly divided into AR group and control group, 10 rats for each group. Ten rats was sensitized and intranasally challenged by ovalbumin and A1 （ OH ） 3 （ as supplement） as allergic rhinitis models, and the control group was treated by saline. RT-PCR was performed to investigate the level of mRNA expression of complement C3 and CA in nasal mueosa of both groups. Results C3 and CA mRNA were detected in both groups. The relative intensity of gene expression was measured. The relative intensity of C3 mRNA expression was 6183 ± 1376 in AR group, n A4A ±989 in control group, CA mRNA was 4398 ± 948 in AR group, and 2771 ±407 in control group. Expression of C3 and CA in AR group was higher than that of the controls （ P 〈 0. 05 ）. Conclusion The high level of C3 and CA mRNA expression in nasal mucosa of rats with allergic rhinitis suggests that C3 and CA are involved in the immunopathology of allergic rhinitis. The result implies that complement system involved in the rat＇s allergic rhinitis is possibly activated through the classical pathway.