乙酰胆碱对大鼠慢性压迫背根节神经元放电的影响

Spontaneous discharge modulation by acetylcholine in chronically compressed rat dorsal root ganglion neurons

目的研究乙酰胆碱(ACh)对大鼠慢性压迫背根节(DRG)神经元放电的影响。方法采用单纤维记录神经元自发放电的方法,在DRG慢性压迫模型上观察ACh对受损DRG神经元自发放电的影响。结果受损DRG神经元有丰富的自发放电,77.9%有自发放电的受损DRG神经元对ACh有反应,其中反应类型有单纯兴奋和先兴奋后抑制两种形式,而且反应的幅度随ACh浓度的延长而逐渐增大。结论在慢性压迫DRG神经元有大量的自发放电,ACh可明显影响受损DRG神经元的自发放电。

Objective To study the effects of acetylcholine （ACh） on spontaneous discharges of compressed rat dorsal root ganglion （DRG） neurons. Methods In chronically compressed DRG model, the spontaneous discharge of a single fiber from the DRG neuron in response to ACh treatment was recorded. Results Active spontaneous discharges were recorded in the injured DRG, and 77.9% of the injured DRG neurons responded to Ach treatment in the manner of simple excitation, or excitation followed by inhibition. The responses were enhanced with the increase of Ach concentration. Conclusion Injured DRG remains active in spontaneous discharges, which can be significantly influenced by ACh treatment.