正常和庆大霉素致耳聋豚鼠清醒和麻醉状态下听性脑干反应的比较

Auditory brainstem response in normal guinea pigs and those with gentamicin-induced hearing loss under awake and anesthetic conditions

目的检测正常豚鼠及庆大霉素致耳聋豚鼠在清醒和麻醉两种状态下的听性脑干反应(ABR),比较两种状态下ABR波形、反应阈及潜伏期等的差别。方法杂色豚鼠40只(正常组和庆大霉素致耳聋组各20只)分别在麻醉前后记录ABR各数据,用配对t检验进行统计学分析。结果正常豚鼠在清醒和麻醉状态下ABR波形,反应阈,Ⅰ、Ⅲ波峰潜伏期,Ⅰ-Ⅲ波峰间期无显著差异(P>0.05);庆大霉素致耳聋豚鼠在清醒和麻醉状态下ABR各参数亦无显著差异(P>0.05)。结论豚鼠(正常或耳聋)在清醒或麻醉状态下测试ABR无显著差异,因此为保证实验成功和减少误差可采用非麻醉方法。

Objective To observe the alterations of auditory brainstem response （ABR） in guinea pigs with gentamicin-induced heating loss under awake and anesthetic conditions. Methods We recorded the ABR in 20 normal guinea pigs and 20 with gentamicin-induced heating loss before and after anesthesia for statistical analysis. Results No significant difference was observed in the waveform, response threshold （RT）, Ⅰ and Ⅲ peak latencies （PL）, Ⅰ -Ⅲ interpeak latencies （IPL） of ABR between awake and anesthetic conditions in normal guinea pigs（P〉0.05）, nor did gentamicin-induced hearing loss showed obvious impact on the ABR parameters （P〉0.05）. Conclusion No significant ABR alterations occur under awake and anesthetic conditions in either normal guinea pigs or those with heating loss, therefore ABR test can be performed without anesthesia to ensure the success and error minimization of the experiment.