结直肠癌组织与血清血管内皮生长因子的相关性

The correlation of serum and tissue vascular endothelial growth factor and human colorectal carcinoma

目的探讨结直肠癌组织与血清血管内皮生长因子(VEGF)的相关性。为结直肠癌的预后判断和治疗后随访寻求一种简便、易测、可靠、无创的检测方法。方法分别应用免疫组化法和ELISA法,测定35例结直肠癌患者的组织和血清VEGF,并与53例非结直肠癌患者作比较。结果①结直肠癌组血清VEGF水平(813.14±511.87)μg/L明显高于对照组(241.40±164.08)μg/L,差异显著(P<0.001);②血清VEGF水平与性别、年龄、肿瘤部位及组织类型无明显相关性(P>0.05);③有淋巴结、肝脏及远处转移者血清和组织VEGF水平比无转移者显著增高(P<0.05;P<0.001);④血清与组织VEGF之间密切相关(P=0.000)。结论①血清和组织VEGF测定能够反映结直肠癌的发展和预后;②结直肠癌患者组织与血清VEGF水平密切相关,血清VEGF可替代组织VEGF的检测。

Purpose Try to find a reliable and atraumatic method to predict the progress and prognosis of human colorectal carcinoma. Methods The vascular endothelial growth factor （VEGF） levels in serums and tissues of 36 patients with colorectal carcinoma were measured by ELISA and immunohistochemistry, meanwhil 53 cases healthy volunteers were also measured as control group. The correlation of serum VEGF and tissue VEGF were especially analyzed. Results ①The level of serum VEGF in patients with colorectal carcinoma was significantly higher than that of control group [（813.14 ± 511.87）μg/L versus （241.40± 164.08）μg/L]. ②Serum VEGF was not correlated to sex, age, tumor site and histological type. ③The levels of serum and tissue VEGF were positively correlated to tumor size, lymph-node involvement and liver or distant organs metastasis. ④Serum VEGF and tissue VEGF were closely correlative. Conclusions ①Since the levels of serum and tissue VEGF were closely correlated with Dukes stage, they can be used as a good parameter to predict development and prognosis of colorectal carcinoma. ②Since the levels of VEGF between serum and tissue were parallel, so serum VEGF measurement may be the substitution of tissue VEGF.