氩氦冷冻对兔肝肿瘤转移的影响

The impact of Argon-Helium cryotherapy on metastasis of rabbit liver tumor

目的探讨氩氦冷冻对兔肝肿瘤转移的影响。方法兔VX2肝肿瘤模型30只随机分为A、B和C组,每组10只,分别给予氩氦冷冻、手术切除和假手术,CT扫描观察肝内播散、肺部和腹腔淋巴结肿瘤转移以及生存期。结果A、B和C组肝内转移率分别为30.0%(3/10),60.0%(6/10)和100.0%(10/10),A组肝内转移率低于C组(P=0.003)。治疗后总体的肺转移率和腹腔淋巴结转移率均为100.0%,但A组肝外转移时间比C组推迟;A组和B组相比,也不促进肿瘤转移。结论氩氦冷冻治疗肝肿瘤不会促进肝内播散或远处转移,和手术切除相比,提示在一定程度上减少肝内播散的机会;氩氦冷冻治疗继发的免疫效应,能抑制已发生的转移灶的生长。

Purpose To investigate the impact of Argon-Helium cryotherapy for rabbit liver cancer on metastasis. Methods Thirty rabbits with VX2 liver carcinoma were divided into group A, B and C randomly （each group n = 10）, they were treated by cryotherapy, hepatic resection and sham operation, respectively. The survival times were analyzed and metastases were observed by CT scans. Results The intrahepatic metastases rates of A, B and C groups were 30%（3/10）, 60%（6/10）, 100%（10/10） respectively. The intrahepatic metastases rate of group A was significantly lower than that of group C（P = 0. 003）. The lung and the peritoneal lymph nodes metastases rates of the 3 group were 100%, and the times of distant metastases of group A were significantly later than those of group C. The rabbits of group A also didn＇t promote metastases in comparison with group B. Conclusions Argon-Helium cryotherapy didn＇t promote intrahepatic dissemination or distant metastasis, and it may suppress intrahepatic spread in comparison to hepatic resection. The immunological effect secondary to cryotherapy could suppress the growth of the metastatic carcinoma.